|
KMID : 0361020000430050463
|
|
Korean Journal of Otolaryngology - Head and Neck Surgery
2000 Volume.43 No. 5 p.463 ~ p.470
|
|
|
Apical Turn Vibratory Response Changes Following Cisplatin Application in the Living Guinea Pig Cochlea
|
|
Jung Ha-Won
Khanna Shyam M.
|
|
|
Abstract
|
|
|
Background and Objectives : Cisplatin (cis-diamminodichloroplatinum) exercises its
ototoxic effect by damaging hair cells of the inner ear. The effect of cisplatin on the
vibratory response of the inner ear was investigated in the apical turn of the cochlea in
11 living guinea pigs.
Materials and Methods : The exposed turn was sealed and fluid coupled to the objective
lens f a slit confocal microscope. Individual cells were identified with the microscope and
the vibration was measured with a confocal heterodyne interferometer. The vibration
was made in response to tones applied to the ear canalat at selected locations such as
the reticular lamina (Hensen cells and outer hair cells), and the basilar membrane
adjacent to the outer Hensen cell edge. Vibration measurements were made before and
after intravenous injections of cisplatin (16 mg/kg).
Results : The drug effect took place approximately 35 minutes after administering
cisplatin. The vibration amplitude changes produced by cisplatin were not reversible with
time. At the level of the reticular lamina, cisplatin reduced the vibration amplitude at
almost all frequencies. However, these effects were small. In contrast to the reticular
lamina response, at the basilar membrane, the vibration amplitude increased following
cisplatin application.
Conclusion : These experiments show that damage to the hair cells in the apical turn of
the cochlea leads to an increase in the basilar membrane vibration. These observations
support the concept of negative feedback in the apical turn of the cochlea.
|
|
|
KEYWORD
|
|
|
Cochlear mechanics, Cisplatin, Reticular lamina, Basilar membrane, Negativefeedback
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|